Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction.
Identifieur interne : 001204 ( Main/Exploration ); précédent : 001203; suivant : 001205Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction.
Auteurs : Jurairat Jongthawin [Thaïlande] ; Pewpan M. Intapan [Thaïlande] ; Viraphong Lulitanond [Thaïlande] ; Oranuch Sanpool [Thaïlande] ; Tongjit Thanchomnang [Thaïlande] ; Lakkhana Sadaow [Thaïlande] ; Wanchai Maleewong [Thaïlande]Source :
- Parasitology research [ 1432-1955 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN des helminthes.
- génétique : Brugia malayi, Wuchereria bancrofti.
- parasitologie : Culicidae.
- Animaux, Chats, Chiens, Filariose lymphatique, Humains, Larve, Mâle, Réaction de polymérisation en chaîne, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Helminth.
- genetics : Brugia malayi, Wuchereria bancrofti.
- methods : Polymerase Chain Reaction.
- parasitology : Culicidae.
- Animals, Cats, Dogs, Elephantiasis, Filarial, Humans, Larva, Male, Sensitivity and Specificity.
Abstract
Lymphatic filariasis, a mosquito-borne disease, is still a major public health problem in tropical and sub-tropical countries. Effective diagnostic tools are required for identification of infected individuals, for epidemiological assessment, and for monitoring of control programs. A duplex droplet digital polymerase chain reaction (ddPCR) was conducted to differentiate and quantify Wuchereria bancrofti DNA by targeting the long DNA repeat (LDR) element and Brugia malayi DNA by targeting the HhaI element in blood samples and mosquito vectors. The analytical sensitivity and specificity were evaluated. Our results indicated that the duplex ddPCR assay could differentiate and quantify W. bancrofti and B. malayi DNA from blood samples and mosquitoes. DNA from a single larva in 50 μl of a blood sample, or in one mosquito vector, could be detected. The analytical sensitivity and specificity for W. bancrofti are both 100 %. Corresponding values for B. malayi are 100 and 98.3 %, respectively. Therefore, duplex ddPCR is a potential tool for simultaneous diagnosis and monitoring of bancroftian and brugian filariasis in endemic areas.
DOI: 10.1007/s00436-016-5051-0
PubMed: 27085707
Affiliations:
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(analyse)</term><term>Animaux</term><term>Brugia malayi (génétique)</term><term>Chats</term><term>Chiens</term><term>Culicidae (parasitologie)</term><term>Filariose lymphatique</term><term>Humains</term><term>Larve</term><term>Mâle</term><term>Réaction de polymérisation en chaîne ()</term><term>Sensibilité et spécificité</term><term>Wuchereria bancrofti (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Helminth</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN des helminthes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Brugia malayi</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Culicidae</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Culicidae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cats</term><term>Dogs</term><term>Elephantiasis, Filarial</term><term>Humans</term><term>Larva</term><term>Male</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chats</term><term>Chiens</term><term>Filariose lymphatique</term><term>Humains</term><term>Larve</term><term>Mâle</term><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lymphatic filariasis, a mosquito-borne disease, is still a major public health problem in tropical and sub-tropical countries. Effective diagnostic tools are required for identification of infected individuals, for epidemiological assessment, and for monitoring of control programs. A duplex droplet digital polymerase chain reaction (ddPCR) was conducted to differentiate and quantify Wuchereria bancrofti DNA by targeting the long DNA repeat (LDR) element and Brugia malayi DNA by targeting the HhaI element in blood samples and mosquito vectors. The analytical sensitivity and specificity were evaluated. Our results indicated that the duplex ddPCR assay could differentiate and quantify W. bancrofti and B. malayi DNA from blood samples and mosquitoes. DNA from a single larva in 50 μl of a blood sample, or in one mosquito vector, could be detected. The analytical sensitivity and specificity for W. bancrofti are both 100 %. Corresponding values for B. malayi are 100 and 98.3 %, respectively. Therefore, duplex ddPCR is a potential tool for simultaneous diagnosis and monitoring of bancroftian and brugian filariasis in endemic areas.</div></front></TEI><affiliations><list><country><li>Thaïlande</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Jongthawin, Jurairat" sort="Jongthawin, Jurairat" uniqKey="Jongthawin J" first="Jurairat" last="Jongthawin">Jurairat Jongthawin</name></noRegion><name sortKey="Intapan, Pewpan M" sort="Intapan, Pewpan M" uniqKey="Intapan P" first="Pewpan M" last="Intapan">Pewpan M. Intapan</name><name sortKey="Lulitanond, Viraphong" sort="Lulitanond, Viraphong" uniqKey="Lulitanond V" first="Viraphong" last="Lulitanond">Viraphong Lulitanond</name><name sortKey="Maleewong, Wanchai" sort="Maleewong, Wanchai" uniqKey="Maleewong W" first="Wanchai" last="Maleewong">Wanchai Maleewong</name><name sortKey="Sadaow, Lakkhana" sort="Sadaow, Lakkhana" uniqKey="Sadaow L" first="Lakkhana" last="Sadaow">Lakkhana Sadaow</name><name sortKey="Sanpool, Oranuch" sort="Sanpool, Oranuch" uniqKey="Sanpool O" first="Oranuch" last="Sanpool">Oranuch Sanpool</name><name sortKey="Thanchomnang, Tongjit" sort="Thanchomnang, Tongjit" uniqKey="Thanchomnang T" first="Tongjit" last="Thanchomnang">Tongjit Thanchomnang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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